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| Status |
Public on Mar 04, 2018 |
| Title |
mRNA expression data from ESCs derived by polar body transfer reconstructed embryos (PBTESCs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we report the derivation of human PBTESCs from polar body transfer resconstructed embryos. We used RNA-seq to compare the gene expression levels among human parthenogenetic haploid ESCs (hPGES)、normal human ESCs (H9) and human forskin fibroblasts and identified that these cells express conventional ESCs pluripotent markers and most maternally imprinted genes were down-regulated.
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| Overall design |
We collected fresh ESCs samples of 3 cell lines from PB1 transfer and 3 cell lines from PB2 transfer each at around 10^6 cells in similar passage. All the sample was lysised by 1 mL Trizol directly and stored at -80℃. DNA libraries building and sequencing was performed at the same time to reduce systematic error. Gene expression profiles of all the cell lines were analysed on illumina Hiseq 2500.
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| Contributor(s) |
Chen Z, Li J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
cuiqing zhong |
| Organization name |
institute of biochemistry and cell biology
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| Street address |
yueyang road 320
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| City |
shanghai |
| ZIP/Postal code |
200010 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA378173 |
| SRA |
SRP101420 |
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