 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 13, 2018 |
| Title |
RNAseq Analysis of Transcriptome expression profiles in E12.5 and E13.5 FACS sorted Osr2RFP/- (mutant)and Osr2RFP/+ (control) palatal mesenchymal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The purpose of this study is to compare the transcriptome expression profiles of E12.5 and E13.5 Osr2RFP/- and Osr2RFP/+ palatal mesenchyme by using RNA-seq analysis.
Methods: Osr2RFP/+ male mice were crossed with Osr2+/- female mice. The embryos were harvested at E12.5 and E13.5. The pair of palatal shelves were dissected from each Osr2-RFP positive embryo. The RFP+ palatal mesenchyme cells were isolated by using fluorescence-activated cell sorting (FACS). RNA-seq analysis was carried out using the FACS-isolated palatal mesenchyme from Osr2RFP/- and Osr2RFP/+ embryos, respectively.
|
| |
|
| Overall design |
The transcriptome expression profiles of E12.5 and E13.5, Osr2RFP/- and Osr2RFP/+ palatal mesenchyme by using RNA-seq analysis, in single pair (E12.5) or duplicates (E13.5), using Illumina HisEq 2000.
|
| |
|
| Contributor(s) |
Xu J, Liu H, Jiang R |
| Citation(s) |
28731788 |
| |
| Submission date |
Mar 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
HAN LIU |
| E-mail(s) |
han.liu@cchmc.org
|
| Organization name |
CINCINNATI CHILDREN'S HOSPITAL
|
| Department |
DEVELOPMENTAL BIOLOGY
|
| Lab |
Jiang
|
| Street address |
3333 burnet avenue
|
| City |
cincinnati |
| State/province |
oh |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA377743 |
| SRA |
SRP101292 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE95638_RAW.tar |
4.8 Mb |
(http)(custom) |
TAR (of XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...