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| Status |
Public on Mar 12, 2017 |
| Title |
Widespread Influence of 3′-end Structures on Mammalian mRNA Processing and Stability [Artificial-3'-end] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding the physiological relevance of structures in mammalian mRNAs remains elusive, especially considering the global unfolding of mRNA structures in eukaryotic organisms recently examined, as well as the decade-long observation that mRNAs generally seem no more likely than random sequences to be stably folded. Here we show that RNA secondary structures, mostly weak and close-to-random, facilitate the 3′-end processing of thousands of human mRNAs by juxtaposing poly(A) signals (PASs) and cleavage sites that are otherwise too far apart. Folding of these 3′-end structures also enhances mRNA stability. Global structure probing shows that 3′-end regions are indeed folded in cells despite substantial unfolding of PAS-upstream regions. Analyses of thousands of ectopically expressed variants prove that folding both enhances processing and increases stability. Mutagenesis of a genomic locus further implicates structure-controlled processing in regulating neighboring gene expression. These results reveal widespread roles for RNA structure in mammalian mRNA biogenesis and metabolism.
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| Overall design |
Two samples: sequencing cleaved and uncleaved variants at a library of artificial poly(A) sites
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| |
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| Contributor(s) |
Wu X, Bartel DP |
| Citation(s) |
28525757 |
| |
| Submission date |
Feb 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Xuebing Wu |
| E-mail(s) |
wuxb07@gmail.com
|
| Phone |
2123047615
|
| Organization name |
Columbia University
|
| Department |
Medicine/Systems Biology
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| Lab |
Xuebing Wu
|
| Street address |
630 West 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE95468 |
Widespread Influence of 3′-end Structures on Mammalian mRNA Processing and Stability |
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| Relations |
| BioProject |
PRJNA377170 |
| SRA |
SRP100808 |
