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| Status |
Public on Aug 31, 2008 |
| Title |
Digging deeper into the PpsR regulon of Rhodobacter sphaeroides |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by array
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| Summary |
The expression of genes involved in photosystem development in Rhodobacter sphaeroides is dependent upon three major regulatory networks: FnrL, the PrrBA two-component system and the AppA-PpsR pathway. A PrrA- mutant strain of R. sphaeroides is unable to develop under photosynthetic conditions, but when combined with a ppsR mutation, is able to resume growth. In an effort to better characterize the ppsR regulon and to unravel the relationship between the PrrBA and the AppA-PpsR networks, we compared transcriptome profiles from the wild type, PrrA- and PrrA-PpsR- strains under a permissive, common, growth condition: anaerobic-dark-DMSO. Here is stored the microarray data for the PrrA-PpsR- double mutant strain grown under this condition.
Keywords: transcriptome profile
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| Overall design |
Rhodobacter sphaeroides PrrA-PpsR- grown under anaerobic dark DMSO conditions. Sparged with 95% N2, 5% CO2 in sistrom minimal medium A supplemented with final concentration 0.1% YE, 0.5% DMSO.
The total RNA from three independent cultures of R. sphaeroides PrrA-PpsR- grown under anaerobic-dark-DMSO conditions was used. cDNA synthesis, fragmentation, labeling and hybridization are described elsewhere (Roh et al., 2004).
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| Contributor(s) |
Bruscella P, Kaplan S |
| Citation(s) |
18689484 |
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| Submission date |
Nov 06, 2007 |
| Last update date |
Apr 26, 2014 |
| Contact name |
patrice bruscella |
| Organization name |
University of Texas Health Science Center
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| Department |
Microbiology and Molecular Genetics
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| Street address |
6431 Fannin
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL162 |
[uwyRhodo1a] Rhodobacter sphaeroides 2.4.1 GeneChip |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA103343 |
