 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2007 |
| Title |
A transcriptional repressor and co-repressor induced by the STAT3-regulated anti-inflammatory signaling pathway. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
IL-10 regulates anti-inflammatory signaling via the activation of STAT3, which in turn controls the induction of a gene expression program whose products execute inhibitory effects on pro-inflammatory mediator production. Here we show that IL-10 induces the expression of an ETS family transcriptional repressor, ETV3 and a helicase family co-repressor, SBNO2 (Strawberry notch homolog 2) in mouse and human macrophages. IL-10-mediated induction of ETV3 and SBNO2 expression was dependent upon both STAT3, and co-stimulus through the TLR pathway. We also observed that ETV3 expression was strongly induced by the STAT3 pathway induced by IL-10 but not STAT3 signaling activated by IL-6, which cannot activate the anti-inflammatory signaling pathway. ETV3 and SBNO2 specifically repressed NF-kB-mediated transcription and can physically interact. Collectively our data suggest that ETV3 and SBNO2 are components of the pathways that contribute to the downstream anti-inflammatory effects of IL-10.
We compared expression profiles of macrophages isolated from IL-10 -/- mice. Macrophages were treated with either LPS or LPS plus IL-10. Treatment times were 10, 20 and 30 minutes.
Keywords: time-course, treatment response, gene expression analysis
|
| |
|
| Overall design |
Mouse IL-10 -/- macrophages were isolated and purified and set up in culture medium containing LPS or LPS plus IL-10. A total of 18 samples were analyzed. This set contains three replicates of each treatment condition where treatment (LPS versus LPS plus IL-10) and time (10min, 20min and 30min) were varied.
|
| |
|
| Contributor(s) |
Murray PJ |
| Citation(s) |
18025162 |
| Submission date |
Nov 02, 2007 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Peter J Murray |
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Infectious Diseases
|
| Street address |
332 Nth Lauderdale
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (18)
|
| GSM241131 |
LPS plus IL-10, 10 min, Rep_1 |
| GSM241132 |
LPS plus IL-10, 10 min, Rep_2 |
| GSM241133 |
LPS plus IL-10, 10 min, Rep_3 |
| GSM241134 |
LPS, 20 min, Rep_1 |
| GSM241135 |
LPS, 20 min, Rep_2 |
| GSM241136 |
LPS, 20 min, Rep_3 |
| GSM241137 |
LPS plus IL-10, 20 min, Rep_1 |
| GSM241138 |
LPS plus IL-10, 20 min, Rep_2 |
| GSM241139 |
LPS plus IL-10, 20 min, Rep_3 |
| GSM241140 |
LPS, 30 min, Rep_1 |
| GSM241141 |
LPS, 30 min, Rep_2 |
| GSM241142 |
LPS, 30 min, Rep_3 |
| GSM241143 |
LPS plus IL-10, 30 min, Rep_1 |
| GSM241144 |
LPS plus IL-10, 30 min, Rep_2 |
| GSM241145 |
LPS plus IL-10, 30 min, Rep_3 |
|
| Relations |
| BioProject |
PRJNA103303 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9509_RAW.tar |
65.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...