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| Status |
Public on Mar 15, 2017 |
| Title |
Myocardial infarction (MI) primes autoreactive T cells through activation of dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DC subsets of the murine healthy heart consisted of IRF8-dependent conventional (c)DC1, IRF4-dependent cDC2 and monocyte-derived DCs. In steady state, cardiac self-antigen α-myosin was presented in heart-draining mediastinal lymph node (mLN) by cDC1s, driving the proliferation of antigen-specific CD4+ TCR-M T cells, and their differentiation into Tregs. Following MI, all DC subsets infiltrated the heart, while only cDCs migrated to the mLN. Here, cDC2s induced TCR-M proliferation and differentiation into IL-17/IFNγ producing effector cells. Thus, cardiac specific autoreactive T cells get activated by mature DCs following myocardial infarction.
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| Overall design |
cDC1s, cDC2s, MFs and moDCs were isolated and sorted from steady state or infarcted hearts in four independent replicates. 31 samples (RNA-Seq) in total. RNA was isolated, amplified by SMARTer amplification, converted to cDNA and then sequenced using Illumina NextSeq.
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| Contributor(s) |
Van der Borght K, Scott CL, Nindl V, Bouché A, Martens L, Sichien D, Van Moorleghem J, Vanheerswynghels M, De Prijck S, Ludewig B, Gillebert T, Guilliams M, Carmeliet P, N Lambrecht B |
| Citation(s) |
28329691 |
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| Submission date |
Feb 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Liesbet Martens |
| E-mail(s) |
liesbet.martens@irc.vib-ugent.be
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| Organization name |
VIB-University of Ghent
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| Department |
VIB Inflammation Research Center
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| Street address |
Technologiepark 927
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| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA374892 |
| SRA |
SRP099882 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94949_RAW.tar |
2.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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