|
| Status |
Public on Aug 30, 2017 |
| Title |
Polycomb Responds to Low Levels of Transcription |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells, as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about 2-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes.
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| |
|
| Overall design |
Five different cell types with five different antibodies.
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| |
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| Contributor(s) |
Berrozpe G, Bryant GO, Warpinski K, Spagna D, Narayan S, Shah S, Ptashne M |
| Citation(s) |
28746865 |
| |
| Submission date |
Feb 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Ptashne |
| E-mail(s) |
m-ptashne@mskcc.org
|
| Organization name |
Sloan Kettering Institute
|
| Department |
Molecular Biology Program
|
| Lab |
Mark Ptashne
|
| Street address |
1275 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (25)
|
|
| Relations |
| BioProject |
PRJNA374889 |
| SRA |
SRP099879 |
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