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| Status |
Public on Jul 06, 2017 |
| Title |
Cell cycle dynamics of chromosomal organisation at single-cell resolution |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Chromosomes in proliferating metazoan cells undergo dramatic structural metamorphoses every cell cycle, alternating between a highly condensed mitotic structure facilitating chromosome segregation, and a decondensed interphase structure accommodating transcription, gene silencing and DNA replication. These cyclical structural transformations have been evident under the microscope for over a century, but their molecular-level analysis is still lacking. Here we use single-cell Hi-C to study chromosome conformations in thousands of individual cells, and discover a continuum of cis-interaction profiles that finely position individual cells along the cell cycle. We show that chromosomal compartments, topological domains (TADs), contact insulation and long-range loops, all defined by ensemble Hi-C maps, are governed by distinct cell cycle dynamics. In particular, DNA replication correlates with build-up of compartments and reduction in TAD insulation, while loops are generally stable from G1 through S and G2. Analysing whole genome 3D structural models using haploid cell data, we discover a radial architecture of chromosomal compartments with distinct epigenomic signatures. Our single-cell data creates an essential new paradigm for the re-interpretation of chromosome conformation maps through the prism of the cell cycle.
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| Overall design |
Hi-C: 45 samples analysed
Strand-specific nuclear RNA-seq: 4 samples analysed
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| Contributor(s) |
Nagano T, Lubling Y, Varnai C, Dudley C, Leung W, Baran Y, Mandelson-Cohen N, Wingett S, Fraser P, Tanay A |
| Citation(s) |
28682332 |
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| Submission date |
Feb 03, 2017 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Steven William Wingett |
| E-mail(s) |
steven.wingett@mrc-lmb.cam.ac.uk
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| Organization name |
MRC Laboratory of Molecular Biology
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| Department |
Cell Biology
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| Street address |
Francis Crick Avenue, Cambridge Biomedical Campus
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| City |
Cambridge |
| State/province |
Cambs |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
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| Platforms (4)
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| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (49)
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| Relations |
| BioProject |
PRJNA369960 |
| SRA |
SRP098829 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE94489_2i_diploids_features_table.txt.gz |
208.6 Kb |
(ftp)(http) |
TXT |
| GSE94489_README.txt |
323.8 Kb |
(ftp)(http) |
TXT |
| GSE94489_haploids_features_table.txt.gz |
121.0 Kb |
(ftp)(http) |
TXT |
| GSE94489_serum_diploids_features_table.txt.gz |
78.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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