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| Status |
Public on Feb 02, 2017 |
| Title |
RNA sequencing of primary human platelets and in vitro cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Platelets are anucleate cytoplasmic fragments that lack genomic DNA, but continue to synthesize protein using a pool of mRNAs, ribosomes, and regulatory small RNAs inherited from the precursor megakaryocyte (MK). The regulatory processes that shape the platelet transcriptome and the full scope of platelet translation have remained elusive. Using RNA-Seq and ribosome profiling of primary human platelets, we show the platelet transcriptome encompasses a subset of transcripts detected by RNA-Seq analysis of in vitro derived MK cells and these platelet-enriched transcripts are broadly occupied by ribosomes. We use RNA sequencing of synchronized populations of in vitro derived platelet-like particles (PLPs) to show that mRNA decay strongly shapes the nascent platelet transcriptome. Our data suggests that the decay of platelet mRNAs is slowed by the natural loss of the mRNA surveillance and ribosome rescue factor Pelota (PELO).
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| Overall design |
Analysis of RNA sequencing data from primary human platelets and in vitro derived platelet-like particles; manipulation by overexpression of Pelota (PELO) in Meg01/platelet-like particles.
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| |
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| Contributor(s) |
Mills EW, Green R, Ingolia NT |
| Citation(s) |
28213379 |
| |
| Submission date |
Feb 01, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric W Mills |
| E-mail(s) |
ewmills@partners.org
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Street address |
725 N. Wolfe St
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA369543 |
| SRA |
SRP098699 |
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