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| Status |
Public on Jan 01, 2018 |
| Title |
Effect of Praziquantel on the Differential Expression of Mouse Hepatic Genes and Parasite ATP Binding Cassette Transporter Gene Family Members During Schistosoma mansoni Infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Schistosomiasis is a disease caused by parasitic worms that significantly impacts on the lives of approximately 250 million people. During infection, female parasites release thousands of eggs, a significant number of which become trapped in the liver. The entrapment of the eggs triggers immune and inflammatory responses that in turn cause many of the health problems associated with the disease. In this study we analyzed the gene expression profiles of livers from mice infected with the human parasite Schistosoma mansoni over multiple time points, beginning when the parasites start to lay eggs. Mice were treated with a lethal dose of the anti-schistosomal drug praziquantel, and we identified a number of different genes and pathways that are central to immune and inflammatory responses that are active even in the absence of egg deposition. Praziquantel is the only drug available to treat schistosomiasis, however it is ineffective against juvenile parasites during the early stages of mammalian infection. We also show that the development of drug resistance may be due to the improved efficacy of the juvenile parasite to actively excrete the drug. These results provide insights into the effect of praziquantel on the host response to infection as well as ability of juvenile parasites to overcome the lethal effect of the drug.
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| Overall design |
Liver mRNA profiles of mice 32, 35, 39, and 46-days post Schistosoma mansoni infection were generated by deep sequencing, in triplicate, using Illumina HiSeq.
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| Contributor(s) |
Cunningham C, Sanchez MC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 27, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Charles Cunningham |
| E-mail(s) |
ccunnin@unm.edu
|
| Organization name |
University of New Mexico
|
| Department |
Biology
|
| Street address |
1 University of New Mexico
|
| City |
Albuquerque |
| State/province |
New Mexico |
| ZIP/Postal code |
87131-0001 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA369030 |
| SRA |
SRP098045 |
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