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| Status |
Public on Jan 17, 2020 |
| Title |
Adult bone marrow-derived progenitors give rise to decidual cells and are essential for implantation and pregnancy maintenance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bone marrow (BM)-derived progenitor cells (BMDPCs) are known to migrate and give rise to endometrial cells however their functional contribution to implantation and pregnancy are unknown . Here we show that implantation and pregnancy are strong stimuli for BMDPCs recruitment to the uterine decidua where BMDPCs differentiate into functional non-hematopoietic prolactin-producing decidual cells. Transcriptome analysis of implantation sites demonstrated that Hoxa11+/- mice transplanted with WT BM clustered hierarchically more closely with normal WT mice than with control Hoxa11+/- mice, showing normalized expression of numerous implantation-related genes. These findings reveal that BMDPCs have a major non-hematopoietic physiologic contribution to the decidual stroma, and thereby play an essential and previously unproven role in the establishment and maintenance of pregnancy.
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| Overall design |
Female Hoxa11 knockout, heterozygotes or wild type mice were given Hoxa11 knockout or wild type bone marrow transplants. After mating, uterine implantation sites were collected and total RNA was extracted on ED 5.5. 14 samples were sequenced by single-end next generation sequencing and differential transcriptome analysis.
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| Contributor(s) |
Tal R, Shaikh S, Liu Y, Pluchino N, Kliman H, Alderman MH, Kayani J, Mammillapalli R, Taylor HS |
| Citation missing |
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| |
| Submission date |
Jan 17, 2017 |
| Last update date |
Jan 17, 2020 |
| Contact name |
Myles H Alderman III |
| Organization name |
Yale University School of Medicine
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| Department |
Cell Biology
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| Street address |
10 Amistad St
|
| City |
New Haven |
| State/province |
Ct |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA362238 |
| SRA |
SRP096918 |
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