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| Status |
Public on Jul 17, 2017 |
| Title |
Detection of miRNA signals in compromised RNA samples |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The aim of these experiments was to clarify the origin of increased expression signals of certain miRNAs in degrading tissues, we found in a previous study.
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| Overall design |
We generated the following RNA samples from liver tissue of three mice (biological replicates) to measure the influence of RNA degradation and DNA contamination on miRNA expression profiles using Agilent microarrays: i) undegraded total RNA including small RNAs without potential DNA contamination, ii) naturally degraded total RNA including small RNAs without potential DNA contamination, iii) undegraded total RNA including small RNAs with potential DNA contamination, iv) naturally degraded total RNA including small RNAs with potential DNA contamination v) undegraded total RNA depleted of small RNAs, vi) partially, in vitro degraded RNA from total RNA depleted of small RNAs, vii) fully degraded, in vitro degraded RNA from total RNA depleted of small RNAs.
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| Contributor(s) |
Ludwig N |
| Citation(s) |
28701729 |
| |
| Submission date |
Jan 13, 2017 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Nicole Ludwig |
| E-mail(s) |
n.ludwig@mx.uni-saarland.de
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| Phone |
004968411626269
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| Organization name |
Saarland University
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| Department |
Human Genetics
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| Street address |
Kirrberger Strasse
|
| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL21265 |
Agilent-070155 Mouse miRNA Microarray (miRBase Release 21.0, miRNA ID version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE93634 |
Effect of RNA integrity on miRNA expression profiles by microarrays and Detection of miRNA signals in compromised RNA samples |
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| Relations |
| BioProject |
PRJNA361321 |
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