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Series GSE92916 Query DataSets for GSE92916
Status Public on Oct 31, 2017
Title Microarray expression data from mouse embryonic stem cells differentiated into Nkx2-1+ lung and thyroid progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary The in vitro directed differentiation of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies.
We used microarrays to detail the global transcriptomes of mouse embryonic stem cells differentiated in vitro into putative thyroid vs lung epithelial lineages using serum-free media supplemented with either BMP4+FGF2 (thyroid media) or BMP4+Wnt3a (lung media.) Differentiated cells carried an Nkx2-1mCherry knock-in reporter to allow sorting of mCherry + vs - cells in each condition on day 14 of differentiation for global transcriptomic profiling.
 
Overall design Mouse embryonic stem cells (carrying an mCherry reporter targeted to the Nkx2-1 3'UTR) were differentiated in serum-free conditions: the cells were first differentiated as embryoid bodies in suspension culture into definitive endoderm with Activin A, followed by differentiation into anterior foregut endoderm by day 6, and then each sample in monolayered 2D culture on gelatin-coated plates was treated on days 6-14 with the growth factor supplements to be tested for each condition (BMP4+FGF2 for thyroid induction media; and BMP4+Wnt3a for lung induction media.)
 
Contributor(s) Serra M, Kotton D
Citation(s) 28947536, 32005814
Submission date Dec 26, 2016
Last update date Feb 10, 2020
Contact name Boston University Microarray and Sequencing Resource
E-mail(s) msrdata@bu.edu
Organization name Boston University
Department Microarray and Sequencing Resource
Street address 72 East Concord Street, E631
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
 
Platforms (1)
GPL17791 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [mogene20st_Mm_ENTREZG_17.1.0]
Samples (12)
GSM2440336 BMP4+FGF2 mCherry- 1
GSM2440337 BMP4+FGF2 mCherry- 2
GSM2440338 BMP4+FGF2 mCherry- 3
Relations
BioProject PRJNA358829

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92916_RAW.tar 114.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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