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| Status |
Public on Jun 25, 2017 |
| Title |
PRC2 specifies ectoderm lineages and maintains pluripotency in primed but not naïve ESCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Polycomb repressive complex 2 and the epigenetic mark that it deposits, H3K27me3, are evolutionarily conserved and play critical roles in development and cancer. However, their roles in cell fate decisions in early embryonic development remain poorly understood. Here we report that knockout of polycomb repressive complex 2 genes in human embryonic stem cells causes pluripotency loss and spontaneous differentiation toward a meso-endoderm fate, owing to de-repression of BMP signalling. Moreover, human embryonic stem cells with deletion of EZH1 or EZH2 fail to differentiate into ectoderm lineages. We further show that polycomb repressive complex 2-deficient mouse embryonic stem cells also release Bmp4 but retain their pluripotency. However, when converted into a primed state, they undergo spontaneous differentiation similar to that of hESCs. In contrast, polycomb repressive complex 2 is dispensable for pluripotency when human embryonic stem cells are converted into the naive state. Our studies reveal both lineage- and pluripotent state-specific roles of polycomb repressive complex 2 in cell fate decisions.
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| Overall design |
We compared the transcriptome of deletion of core components of PRC2 in human embryonic stem cells and their wildtype counterparts by RNA-Seq.
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| Contributor(s) |
Shan Y, Chen Q, Pan G, Du J, Yang Z, Hutchins A |
| Citation(s) |
28939884 |
| |
| Submission date |
Dec 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
qianyu chen |
| E-mail(s) |
chen_qianyu@gibh.ac.cn
|
| Phone |
+8602032015236
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Lab |
Guangjin Pan
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (19)
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| Relations |
| BioProject |
PRJNA358193 |
| SRA |
SRP095403 |
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