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| Status |
Public on Sep 02, 2019 |
| Title |
Expression profiling of mouse primary prostate cells from WT mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
By single-cell expression profiling and validation, here we report that both basal and luminal-localized cells, particularly the latter, are molecularly heterogeneous. We validate LY6D as a marker linking CR luminal cells to luminal progenitors.
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| Overall design |
Primary prostate cells were isolated and luminal prostate cells were FACS-sorted by LY6D+/- SCA1High/Int/low from the lineage (CD31, CD45, Ter119) negative fraction. Total RNA was extracted from two biological replica test. Lexogen Quantseq RNA Sample Prep Kit was used with 10-20 ng of total RNA for the construction of sequencing libraries.
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| Contributor(s) |
Baena E |
| Citation(s) |
30566873 |
| |
| Submission date |
Dec 20, 2016 |
| Last update date |
Sep 22, 2023 |
| Contact name |
Esther Baena |
| E-mail(s) |
Esther.baena@cruk.manchester.ac.uk
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Department |
Prostate Oncobiology
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| Lab |
Esther Baena
|
| Street address |
The University of Manchester, Wilmslow Road
|
| City |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA358189 |
| SRA |
SRP095399 |
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