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| Status |
Public on Dec 20, 2016 |
| Title |
Mapping of DHT-responsive or -independent AR-binding sites induced by activated Src in prostate cancer cell lines [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Building on the observation that metastatic, castration-resistant prostate cancer (CRPC) correlates with activation of Src-family tyrosine kinases, we showed that the expression of activated Src renders LNCaP androgen-independent. Here, we report on RNA-seq and/or AR ChIP-seq analyses of LNCaP, LNCaP[Src], VCaP, 22Rv1 cells grown in the presence or absence of 10 nM DHT for 16h, or LuCaP35.1 tumors grown in androgen-supplemented vs. castrated mice (androgen-dependent vs. castration-resistant). We identify an 11-gene Src-induced signature found only in CRPC in response to DHT, and moreover, the differentail expression of a subset (DPP4, BCAT1, CNTNAP4, CDH3) correlates with earlier PC metastasis onset and poorer survival.
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| Overall design |
RNA-seq of NGS libraries and sequencing of prostate cancer cell lines. 16 samples are seqeunced. For VCaP, LnCap, LnCap-SRC, 22RV1, and LNCap-C4-2, we have both DHT treated and control cell lines. For LuCaP 35.1 cell line, xenografted scid mice tumors are seqeucned, 3 androgen-dependent and 3 castration-recurrent samples ar sequenced.
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| |
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| Contributor(s) |
Gelman IH |
| Citation(s) |
28055971 |
| |
| Submission date |
Dec 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jianmin Wang |
| E-mail(s) |
Jianmin.Wang@roswellpark.org
|
| Organization name |
Roswell Park Comprehensive Cancer Center
|
| Department |
Bioinformatics and Biostatistics
|
| Street address |
Elm and Carlton Streets
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE92576 |
Mapping of DHT-responsive or -independent AR-binding sites induced by activated Src in prostate cancer cell lines |
|
| Relations |
| BioProject |
PRJNA358067 |
| SRA |
SRP095351 |
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