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| Status |
Public on Dec 21, 2018 |
| Title |
Microarray expression profiling data for sorted prostate epithelial subpopulations based on LY6D |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In the normal prostate, most basal and some luminal cells are castration-resistant (CR). The identity of these CR cells and their relation to CR prostate cancer are unresolved. We compared single-cell expression profiles of prostate cells sorted from hormonally naïve (HN) and castrated mice. We found both basal and luminal-localized cells, particularly the latter, were molecularly heterogeneous. CR luminal cells and a subset of HN luminal cells exhibited a similar “intermediate” expression pattern, including high-level expression of multiple prostate stem/progenitor marker genes and androgen receptor gene. We validated LY6D as a marker linking CR luminal cells to luminal progenitors. LY6D+ prostate cells, including LY6D+ luminal cells, were enriched for organoid-forming potential regardless of the presence or absence of androgen. Krt8-based lineage-tracing revealed that LY6D+ CR luminal cells produced LY6D- normal luminal cells upon regeneration, but LY6D+ luminal cancer cells under PTEN-deficiency. Furthermore, prostate cancers originating from CR luminal cells (LY6D+) exhibited a more advanced phenotype than those from HN luminal cells (LY6D+ or LY6D-). Lastly, LY6D amplification/upregulation appear associated with advanced prostate cancer in patient samples. Together, our studies demonstrate LY6D as a novel progenitor marker predictive of lethal CR disease.
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| Overall design |
Subpopulations of prostate epithelial cells based on Sca1, CD49f, LY6D and lineage markers (negative for CD45, CD31, Ter119) were sorted from wild-type hormone-naïve mice; total RNAs were prepared from each sorted sample and subjected to microarray expression profiling.
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| Contributor(s) |
Pakula H, Li Z |
| Citation(s) |
30566873 |
| |
| Submission date |
Dec 15, 2016 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Zhe Li |
| E-mail(s) |
zli4@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine, Genetics Division
|
| Lab |
Li Lab
|
| Street address |
77 Avenue Louis Pasteur, Room 466
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (12)
|
| GSM2430387 |
LY6D+ prostate luminal epithelial cells from mouse #4 |
| GSM2430388 |
LY6D+ prostate luminal epithelial cells from mouse #5 |
| GSM2430389 |
LY6D- prostate luminal epithelial cells from mouse #4 |
| GSM2430390 |
LY6D- prostate luminal epithelial cells from mouse #5 |
| GSM2430391 |
LY6D+ Sca1-high prostate epithelial cells from mouse #1 |
| GSM2430392 |
LY6D+ Sca1-high prostate epithelial cells from mouse #5 |
| GSM2430393 |
LY6D- Sca1-high prostate epithelial cells from mouse #1 |
| GSM2430394 |
LY6D- Sca1-high prostate epithelial cells from mouse #5 |
| GSM2430395 |
LY6D+ prostate basal epithelial cells from mouse #3 |
| GSM2430396 |
LY6D+ prostate basal epithelial cells from mouse #5 |
| GSM2430397 |
LY6D- prostate basal epithelial cells from mouse #3 |
| GSM2430398 |
LY6D- prostate basal epithelial cells from mouse #5 |
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| Relations |
| BioProject |
PRJNA357633 |
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