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| Status |
Public on May 02, 2017 |
| Title |
MR-dependent transcriptional regulation of IWAT, BAT, Muscle, and Liver Gene Expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 36 trimethylation marks primary coding and non-coding transcripts, facilitating gene annotation. Trimethylation of lysine 9 and lysine 20 is detected at satellite, telomeric and active long-terminal repeats, and can spread into proximal unique sequences. Lysine 4 and lysine 9 trimethylation marks imprinting control regions. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations and transcription effects of dietary methionine restriction in peripheral tissues.
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| Overall design |
Examination of transcriptional effects of Control and Methionine-restricted diets across 4 tissues
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| Contributor(s) |
Gettys TW, Forney LA, Wanders D, Stone KP, Ghosh S |
| Citation(s) |
28261952, 28520765 |
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| Submission date |
Dec 15, 2016 |
| Last update date |
Sep 02, 2019 |
| Contact name |
Thomas W Gettys |
| E-mail(s) |
gettystw@pbrc.edu
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| Phone |
225-763-3165
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| Organization name |
Pennington Biomedical Research Center
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| Department |
Nutrient Sensing and Adipocyte Signaling
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| Street address |
6400 Perkins Road
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| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
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| Platforms (1) |
| GPL15907 |
AB 5500xl Genetic Analyzer (Mus musculus) |
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| Samples (47)
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| Relations |
| BioProject |
PRJNA357615 |
| SRA |
SRP095201 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92463_BAT_DESeq_NormCts.txt.gz |
558.7 Kb |
(ftp)(http) |
TXT |
| GSE92463_BAT_RawCts.txt.gz |
343.7 Kb |
(ftp)(http) |
TXT |
| GSE92463_Liver_DESeq_NormCts.txt.gz |
533.9 Kb |
(ftp)(http) |
TXT |
| GSE92463_Liver_RawCts.txt.gz |
343.4 Kb |
(ftp)(http) |
TXT |
| GSE92463_SM_DESeq_NormCts.txt.gz |
532.1 Kb |
(ftp)(http) |
TXT |
| GSE92463_SM_RawCts.txt.gz |
325.0 Kb |
(ftp)(http) |
TXT |
| GSE92463_iWAT_DESeq_NormCts.txt.gz |
599.3 Kb |
(ftp)(http) |
TXT |
| GSE92463_iWAT_RawCts.txt.gz |
342.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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