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| Status |
Public on Sep 18, 2017 |
| Title |
Identification of imprinted gene Grb10 to associate with the pluripotency state in nuclear transfer embryonic stem cells using high throughput sequencing technology |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We compared the exactly syngeneic ntESCs and iPSCs with same genomic insertion generated from adipocyte progenitor cells (APCs) isolated from the all-iPSC mice through the primary TF mediated reprogramming by performing the high-throughput sequencing. There were 84 genes significantly upregulated in fully reprogrammed ntESCs compared with partially reprogrammed ntESCs and 391 genes upregulated in fully reprogrammed iPSCs compared with partially reprogrammed iPSCs. An overlapping gene, Grb10, was identified to associate with the pluripotency state of ntESCs.
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| |
|
| Overall design |
Examination of the mRNA expression in different cell types.
|
| |
|
| Contributor(s) |
Li H, Han Z, Gao S |
| Citation(s) |
28476045 |
| |
| Submission date |
Dec 13, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
huanwei huang |
| E-mail(s) |
hhw2436@gmail.com
|
| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (16)
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|
| Relations |
| BioProject |
PRJNA357194 |
| SRA |
SRP095002 |
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