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| Status |
Public on Dec 13, 2016 |
| Title |
Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Increasing evidence supports the idea that cancer cell plasticity promotes metastasis and tumor recurrence, resulting in patient mortality. While it is clear that the tumor microenvironment (TME) contributes to cancer cell plasticity, the specific TME factors most actively controlling plasticity remain largely unknown. Here, we performed a screen to identify TME cytokines and growth factors that promote epithelial-mesenchymal plasticity, and acquisition of cancer stem-cell (CSC) properties. Of 28 TME cytokines and growth factors tested, we identified Oncostatin M (OSM) as the most potent inducer of mesenchymal/CSC properties. OSM-induced plasticity was Signal Transducer and Activator of Transcription 3 (STAT3)-dependent, and also required a novel intersection with Transforming Growth Factor-β (TGF-β)/SMAD signaling. OSM/STAT3 activation promoted SMAD3 nuclear accumulation, DNA-binding, and induced SMAD3-dependent transcriptional activity. Suppression of TGF-β receptor activity or ablation of SMAD3 or SMAD4, but not SMAD2, strongly suppressed OSM/STAT3-mediated plasticity. Moreover, removal of OSM or inhibition of STAT3 or SMAD3 resulted in a marked reversion to a non-invasive, epithelial phenotype. We propose that, targeted blockade of the STAT3/SMAD3 axis in tumor cells may represent a novel therapeutic approach to prevent the plasticity required for metastatic progression and tumor recurrence.
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| Overall design |
Total RNA was isolated from sorted CD24+ 48R-shp16-shp53-MYC-RAS which were cultured under normal conditions (CD24+ NT) or in the presence of human recombinant OSM 10ng/mL (CD24+ OSM) or human recombinant TGF-b 10ng/mL (CD24+ TGF-b) for three weeks. Cells were treated at every medium change.
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| Contributor(s) |
Jackson MW |
| Citation(s) |
28288136 |
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| Submission date |
Dec 12, 2016 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Mark Jackson |
| E-mail(s) |
mark.w.jackson@case.edu
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| Phone |
2163681276
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| Organization name |
Case Western Reserve University
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| Department |
Pathology
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| Lab |
Mark Jackson
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| Street address |
10900 Euclid Ave
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA357114 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92297_RAW.tar |
31.0 Mb |
(http)(custom) |
TAR (of IDAT) |
| GSE92297_non-normalized.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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