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| Status |
Public on Jan 31, 2018 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of Wild Type and MEIS1 deleted H1 drived cells at day6 of megakaryocytic differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to investigate the molecular mechanism by which MEIS1 controls megakaryocytic maturation and thrombopoiesis through compareing the mRNA profiling of Wild Type and MEIS1 deleted H1 drived cells at day6 of megakaryocytic differentiation.
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| Overall design |
Methods: mRNA profiles of Wild Type and MEIS1 deleted H1 drived cells at day6 of megakaryocytic differentiation. were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
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| Contributor(s) |
Zhou J, Wang H |
| Citation(s) |
29358086, 37378343 |
| |
| Submission date |
Dec 12, 2016 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Jiaxi Zhou |
| E-mail(s) |
leisheng_zhang@163.com
|
| Phone |
+86-22-23909101
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| Organization name |
PUMC
|
| Street address |
288# Nanjing Road, He ping district
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE92245 |
MEIS1 regulates hematopoiesis in hPSCs |
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| Relations |
| BioProject |
PRJNA357018 |
| SRA |
SRP094926 |
