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| Status |
Public on Jan 31, 2018 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of Wild Type and MEIS1 deleted H1 drived cells at day3 of hematopoietic differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study are to investigate the molecular mechanism by which MEIS1 controls HEP specification through compareing the mRNA profiling of Wild Type and MEIS1 deleted H1 drived cells at day3 of hematopoietic differentiation.
Conclusions:a large number of genes were down-regulated in MEIS1-deleted H1 hESCs when compared with the wild-type cells. Among those, a number of mammalian hematopoiesis-associated genes such as FLI1, APLN, TAL1 and MYB were significantly down-regulated.
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| Overall design |
Methods: mRNA profiles of Wild Type and MEIS1 deleted H1 drived cells at day3 of hematopoietic differentiation. were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
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| Contributor(s) |
Zhou J, Wang H |
| Citation(s) |
29358086, 37378343 |
| |
| Submission date |
Dec 12, 2016 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Jiaxi Zhou |
| E-mail(s) |
leisheng_zhang@163.com
|
| Phone |
+86-22-23909101
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| Organization name |
PUMC
|
| Street address |
288# Nanjing Road, He ping district
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE92245 |
MEIS1 regulates hematopoiesis in hPSCs |
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| Relations |
| BioProject |
PRJNA357017 |
| SRA |
SRP094925 |
