|
| Status |
Public on Dec 19, 2017 |
| Title |
Comparing gene expression in iPSC derived RPE from control and AMD donors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
iPSC-RPE cells derived from a patient with AMD, whom harboured the Y402H mutation were compared to healthy donor RPE cells.
|
| |
|
| Overall design |
RPE cells cultured for 6 months on hanging inserts were dissociated with TryPLE, cells were centrifuged at high speed for 3 minutes, TryPLE solution was aspirated. The cell pellet was then re-suspended in PBS. Cells were centrifuged a second time, PBS was removed and cell pellets were snap frozen on dry ice and stored at -80c until use
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| |
|
| Contributor(s) |
Lako M, Hallam D |
| Citation(s) |
28913923 |
| |
| Submission date |
Dec 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Dean Mark Hallam |
| E-mail(s) |
dean.hallam@ncl.ac.uk
|
| Organization name |
Newcastle University
|
| Department |
Institute of Genetic Medicine
|
| Street address |
International Centre for Life
|
| City |
Newcastle upon Tyne |
| State/province |
Tyne and Wear |
| ZIP/Postal code |
NE1 3BZ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA356843 |
| SRA |
SRP094838 |
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