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| Status |
Public on Jan 16, 2017 |
| Title |
Tethered Oligonucleotide-Primed sequencing (TOP-seq): a high resolution economical approach for DNA epigenome profiling |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We describe the development and thorough validation of a new approach for epigenome analysis (named TOP-seq) that is based on a novel concept – combination of selective covalent tagging and targeted genomic sequencing of the genome. The technique reports the modification status of each genomic CpG site by priming the DNA polymerase action from a tethered oligonucleotide probe that is selectively attached to the unmodified CpG sites.
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| Overall design |
DNA methylation analysis of 4 human tissues/cell lines (Prefrontal brain cortex - 4 replicates; IMR90 - 9 replicates; Neuroblastoma specific LA1-55n - 3 replicates; Non-neuronal progenitor LA1-5s - 3 replicates)
Please note that processed files created by merging data from multiple samples are linked as Series supplementary file and are indicated in the corresponding Sample description field.
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| Contributor(s) |
Staševskij Z, Gibas P, Gordevičius J, Kriukienė E, Klimašauskas S |
| Citation(s) |
28111014 |
| |
| Submission date |
Dec 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Saulius Klimašauskas |
| Organization name |
Vilnius University
|
| Department |
Department of Biological DNA Modification, Institute of Biotechnology
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| Street address |
Sauletekio al. 7
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| City |
Vilnius |
| ZIP/Postal code |
LT-10257 |
| Country |
Lithuania |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA356692 |
| SRA |
SRP094749 |
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