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| Status |
Public on Nov 27, 2017 |
| Title |
FTY720 (fingolimod) regulates key target genes essential for inflammation in microglial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Although microglial have an essential role in host defense in the brain, the abnormal activation of microglia can lead to devastating outcomes, such as neuroinflammation and neurodegeneration. Emerging evidence increasingly supports that FTY720 (fingolimod), an FDA-approved drug has beneficial effects in the CNSÂ on brain cells and more recently immunosuppressive activities in microglia via modulation of the S1P1 receptor. However, the exact molecular aspects of FTY720 contribution in microglia remain largely unaddressed. To understand the molecular mechanisms underlying the roles of FTY720 in microglia, we performed gene expression profiling in resting, FTY720, LPS and LPS+FTY720 challenged primary microglial cells (PM) isolated from 3-day-old ICR mice and we identified FTY720 target genes and co-regulated modules that were critical in inflammation. By examining RNA-sequencing and binding motif datasets from FTY720 suppressed LPS-induced inflammatory mediators, we also identified unexpected relationships between the inducible transcription factors (TFs), motif strength, and transcription of key inflammatory mediators. Furthermore, we show that FTY720 controls important inflammatory genes targets by modulating STAT1, and IRF8 level at their promoter site. Our unprecedented findings demonstrate that FTY720 could be a useful therapeutic application for neuroinflammatory diseases associated with microglia activation as well as provide a rich resource and framework for future analyses of FTY720 on microglia interaction.
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| Overall design |
Murine microglial PM, were treated with FTY720 (1 uM), and with LPS (10 ng/ml) for 4 hrs.
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| Contributor(s) |
Chai Y |
| Citation(s) |
28373076 |
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| Submission date |
Dec 06, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Young Gyu Chai |
| E-mail(s) |
ygchai@hanyang.ac.kr
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| Phone |
+82-31-400-5513
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| Organization name |
Hanyang Univ.
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| Department |
Molecualr & Life Science
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| Street address |
55 Hanyangdaehak-ro
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| City |
Ansan |
| State/province |
Gyeonggi-do |
| ZIP/Postal code |
15588 |
| Country |
South Korea |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA356394 |
| SRA |
SRP094625 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE90932_RAW.tar |
880.0 Kb |
(http)(custom) |
TAR (of TAB) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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