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| Status |
Public on Feb 03, 2017 |
| Title |
Effect of 48h treatment with 100nM GSK2879552 on T-ALL cell lines HSB2 and RPMI8402 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA sequencing was performed on biological triplicates of HSB2 and RPMI8402 cells treated with 100nM GSK2879552 for 48h versus their corresponding DMSO treatment controls. GSK2879552 is a potent, selective and orally bioavailable inhibitor of the lysine-specific histone demethylase 1 (LSD1). Gene expression was severely affected by LSD1 inhibition. Pathway analysis performed on the differentially expressed genes following 48hrs of LSD1i treatment clearly showed an increased apoptotic gene signature (CASP3, CASP8) and enhanced expression of genes associated with TRAIL signaling. Furthermore, pre-ranked Gene Set Enrichment Analysis (GSEA) revealed that genes upregulated after LSD1 inhibition in HSB2 showed significant enrichment for transcripts that are down in hematopoietic stem cells and early T lymphocytes. In contrast, important components of the Notch, WNT and SMAD signalling complex were significantly down regulated upon short-term LSD1i treatment in HSB2 cells. In line with this notion, pre-ranked GSEA also revealed that LSD1i resulted in decreased expression of genes that significantly overlap with an oncogenic MYC signature.
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| Overall design |
HSB2 and RPMI8402 cells were treated with 100nM GSK2879552 or DMSO for 48h. Three biological replicates of this treatment were performed for each celline.
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| Contributor(s) |
Peirs S, Goossens S, Van Loocke W, Van Vlierberghe P, Haigh J |
| Citation(s) |
28069602 |
| |
| Submission date |
Nov 29, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sofie Peirs |
| E-mail(s) |
sofie.peirs@ugent.be
|
| Organization name |
Ghent University
|
| Street address |
De Pintelaan 185
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE83128 |
Oncogenic ZEB2 activation drives sensitivity towards LSD1 inhibition in T-cell acute lymphoblastic leukemia |
|
| Relations |
| BioProject |
PRJNA355292 |
| SRA |
SRP094062 |
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