NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE90508 Query DataSets for GSE90508
Status Public on Dec 31, 2017
Title Neuronal activity, TGFβ signaling and unpredictable chronic stress affect transcription of Gadd45 family members in vitro and in vivo [Affymetrix]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Neuronal activity is altered in several neurological and psychiatric diseases. Upon depolarization not only neurotransmitters are released but also cytokines and other activators of signaling cascades. Unraveling their complex implication in transcriptional control in receiving cells will contribute to understand specific central nervous system (CNS) pathologies and will be of therapeutical interest. In this study we depolarized mature hippocampal neurons in vitro using KCl and revealed increased release not only of Brain-derived neurotrophic factor (BDNF), but also of Transforming growth factor beta (TGFB). Neuronal activity together with BDNF and TGFB controls transcription of DNA modifying enzymes specifically members of the DNA-damage-inducible (Gadd) family, Gadd45a, Gadd45b, and Gadd45g. MeDIP followed by massive parallel sequencing and transcriptome analyses revealed less DNA methylation upon KCl treatment. Thereby, psychiatric disorder-related genes, namely Tshz1, Foxn3, Jarid2, Per1, Map3k5, and Arc are transcriptionally activated and demethylated upon neuronal activation. To analyze whether misexpression of Gadd45 family members can be associated with psychiatric diseases, we applied unpredictable chronic mild stress (UCMS) as established model for depression to mice.UCMS lead to a reduced expression of Gadd45 family members. Taken together, our data demonstrate that Gadd45 family members are new putative targets for UCMS treatments.
 
Overall design Definition of activity dependent targets on transcriptional and DNA methylation level
 
Contributor(s) Franz H, Bovio P, Grassi D, Vogel T, Vezzali R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 25, 2016
Last update date Feb 21, 2018
Contact name Henriette Franz
Organization name University Freiburg
Department Anatomy and Cellbiology
Lab Vogel
Street address Alberstr: 17
City Freiburg
ZIP/Postal code 79104
Country Germany
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (8)
GSM2405847 Hippocampal_neurons_untreated_rep1
GSM2405848 Hippocampal_neurons_4h_KCL_rep1
GSM2405849 Hippocampal_neurons_untreated_rep2
This SubSeries is part of SuperSeries:
GSE90513 Neuronal activity, TGFβ signaling and unpredictable chronic stress affect transcription of Gadd45 family members in vitro and in vivo
Relations
BioProject PRJNA354945

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90508_RAW.tar 73.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap