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Series GSE90459 Query DataSets for GSE90459
Status Public on Nov 24, 2017
Title RNA-seq analysis of mouse brain endothelial cells and glioma endothelial cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Glioblastoma multiforme (GBM) is a highly aggressive and vascularized malignant brain tumor. Anti-VEGF therapy is widely used for the treatment of GBM, however it has shown only minor impact on patient survival. Thus, more precise molecular mechanisms for glioma angiogenesis are needed for the advance in the treatment of GBM. Here, we investigated gene expression profile of brain endothelial cells and glioma endothelial cells using RNA sequencing to validated special molecular features of glioma endothelial cells.
Overall design To reveal molecular mechanisms underlying vascular characteristics of high-grade glioma, we established mouse orthotopic glioma model by intracranially injecting GL26l glioma cells into congenic B6 genetic background mice. We further compared tumor vessels with non-tumor brain vessels at the molecular level. For in-depth understanding of different gene expression between tumor vessels and brain vessels, we purified endothelial population from tumor and brain tissues and performed RNA-sequencing analysis. Three independent sets of RNA were obtained from normal brain endothelial cells and glioma endothelial cells.
Contributor(s) Kim K, Kim I, Kim I
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Submission date Nov 23, 2016
Last update date May 15, 2019
Contact name KANGSAN KIM
Phone +1-214-906-0923
Organization name UT Southwestern Medical Center
Department Pathology
Lab Malladi Lab
Street address 5323 Harry Hines Blvd
State/province TX
ZIP/Postal code 75290
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2401523 Normal brain EC #1
GSM2401524 Normal brain EC #2
GSM2401525 Normal brain EC #3
BioProject PRJNA354698
SRA SRP093818

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Supplementary file Size Download File type/resource
GSE90459_RAW.tar 700.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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