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| Status |
Public on Nov 24, 2017 |
| Title |
RNA-seq analysis of mouse brain endothelial cells and glioma endothelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Glioblastoma multiforme (GBM) is a highly aggressive and vascularized malignant brain tumor. Anti-VEGF therapy is widely used for the treatment of GBM, however it has shown only minor impact on patient survival. Thus, more precise molecular mechanisms for glioma angiogenesis are needed for the advance in the treatment of GBM. Here, we investigated gene expression profile of brain endothelial cells and glioma endothelial cells using RNA sequencing to validated special molecular features of glioma endothelial cells.
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| Overall design |
To reveal molecular mechanisms underlying vascular characteristics of high-grade glioma, we established mouse orthotopic glioma model by intracranially injecting GL26l glioma cells into congenic B6 genetic background mice. We further compared tumor vessels with non-tumor brain vessels at the molecular level. For in-depth understanding of different gene expression between tumor vessels and brain vessels, we purified endothelial population from tumor and brain tissues and performed RNA-sequencing analysis. Three independent sets of RNA were obtained from normal brain endothelial cells and glioma endothelial cells.
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| Contributor(s) |
Kim K, Kim I, Kim I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
KANGSAN KIM |
| E-mail(s) |
Kangsan.kim@utsouthwestern.edu
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| Phone |
+1-214-906-0923
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| Organization name |
UT Southwestern Medical Center
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| Department |
Pathology
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| Lab |
Malladi Lab
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| Street address |
5323 Harry Hines Blvd
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| City |
DALLAS |
| State/province |
TX |
| ZIP/Postal code |
75290 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA354698 |
| SRA |
SRP093818 |
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