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| Status |
Public on Dec 15, 2016 |
| Title |
Comparison of bovine blastocysts (192 hpi) embryo transcriptome in presence of co-culture systems (BOEC or VERO) in SOF medium |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
To analyze impact of co-culture presence and type of feeder cell lines, we compared the transcriptome of bovine blastocyst (192 hpi) using a new custom bovine microarray.
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| Overall design |
In vitro produced putative zygotes were cultured in 500 µl weel (25 embryos per well) in control condition (5% O2, 5%, 3 replicates), in high level of oxygen (20% O2, 20%, 3 replicates), in presence of BOEC at 20%O2 (BOEC, 3 replicates) or in presence of VERO (VERO, 4 replicates). Embyos were sampled at 16-cell stage (96 hours post insemination).
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| Contributor(s) |
Vitorino Carvalho A, Duranthon V |
| Citation missing |
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| |
| Submission date |
Nov 22, 2016 |
| Last update date |
Dec 17, 2016 |
| Contact name |
Anaïs Vitorino Carvalho |
| E-mail(s) |
anais.carvalho@hotmail.fr
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| Organization name |
INRA
|
| Department |
BDR
|
| Street address |
Domaine de Vilvert
|
| City |
Jouy-en-Josas |
| ZIP/Postal code |
78350 |
| Country |
France |
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| Platforms (1) |
| GPL22702 |
Agilent-075257 Bovine Microarray FECUND INRA v2 022520 [PROBE NAME] |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE90473 |
Comparison of bovine embryo transcriptome at 16-cell (96 hpi) and blastocysts (192 hpi) stages in presence of co-culture systems (BOEC or VERO) in SOF medium |
|
| Relations |
| BioProject |
PRJNA354639 |
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