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| Status |
Public on Jun 26, 2019 |
| Title |
Epithelial-mesenchymal transition markers screenedina cell-based model and validated in lung adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The epithelial-to-mesenchymal transition (EMT) process in cancer enables the migratory and invasive capabilities associated with metastatic competence. The cancer microenvironment plays an important role in inducing the occurrence of EMT. A number of extracellular stimuli and associated signaling pathways have been identified to promote EMT, however, molecular features during the early stages of EMT are poorly understood. Here we have established a novel in vitro EMT-inducing system that mimi cs tumor microenvironment and allows for close monitoring of the EMT progress. Using RNA-seq and miRNA-seq, we analyzed the transcriptomic transitions accompanying the progression of EMT, and pinpointed several molecular features during early EMT of the lung carcinoma cells.
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| Overall design |
CAF conditioned medium treated and control A549 cells were collected at six different time points (3h, 6h, 12h, 24h, 48h, 72h) for RNA-seq and miRNA-seq, two biological replicates for each sample.
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| Contributor(s) |
Song J, Wang W, Zhang Y, Wang Q |
| Citation missing |
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| |
| Submission date |
Nov 22, 2016 |
| Last update date |
Jun 26, 2019 |
| Contact name |
dong chen |
| Organization name |
ablife
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| Street address |
GaoXin 2nd Road
|
| City |
wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430064 |
| Country |
China |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA354518 |
| SRA |
SRP093710 |
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