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| Status |
Public on Feb 14, 2017 |
| Title |
Analysis of transcriptional and epigentic changes induced by loss of Phf8 [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Phf8 is a histone demethylase with links to human behavior. Here we have performed a transcriptional analysis of Phf8 knockout embryonic stem and neural projenitor cells in vitro along with an analysis of the prefrontal cortex and ventral striatum in vivo. Our results suggest that knockout of Phf8 is largely compensated for. However, we did observe a misregulation of serotonin receptors in the prefrontal cortex, which may have disease implications.
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| Overall design |
mRNA was generated from WT and KO ESCs and NPCs in culture or from prefrontal corteces and ventral striata dissected from adult WT or KO mice.
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| Contributor(s) |
Hochedlinger K, Walsh RM |
| Citation(s) |
28485378 |
| |
| Submission date |
Nov 17, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ryan Walsh |
| E-mail(s) |
walshr1@mskcc.org
|
| Organization name |
Harvard
|
| Lab |
Hochedlinger
|
| Street address |
185 Cambridge St. CPZN 4100
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE90020 |
Analysis of histone methylation in the neocortex of Phf8 KO mice, and transcriptional/epigentic changes induced by loss of Phf8 |
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| Relations |
| BioProject |
PRJNA354483 |
| SRA |
SRP093797 |
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