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Series GSE8993 Query DataSets for GSE8993
Status Public on Sep 02, 2008
Title Normal human fibroblast cell line bystander effects by Carbon ion irradiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We have recently investigated the response of irradiated and bystander fibroblasts to heavy ions in confluent cultures exposed randomly to broadbeam and selectively to precision microbeam that target a predefined fraction of cells with counted number of particle(s), respectively, and found the difference in the time course of apoptosis induction and p53 phosphorylation between irradiated and bystander cells [Hamada MR2008]. In the present investigation, to further scrutinize the underpinnings of their temporally distinct responses, we set out to examine the gene expression changes in irradiated and bystander fibroblasts at a genome-wide level with the microarray analysis under the same experimental condition. We demonstrate that the gene expression profiles of bystander cells are substantially different from those of heavy ion-irradiated cells.
Keywords: bystander effect, hour-time course cell number dependency, irradiation-dose response
Overall design 36 samples ; Broadbeam control A, Broadbeam control B, Broadbeam sham 2 h A, Broadbeam sham 2 h B, Broadbeam sham 6 h A, Broadbeam sham 6 h B, Broadbeam D 2 h A, Broadbeam D 2 h B, Broadbeam D 6 h A, Broadbeam D 6 h B, Broadbeam 0.1D 2 h A, Broadbeam 0.1D 2 h B, Broadbeam 0.1D 6 h A, Broadbeam 0.1D 6 h B, Broadbeam 0.01D 2 h A, Broadbeam 0.01D 2 h B, Broadbeam 0.01D 6 h A, Broadbeam 0.01D 6 h B, Microbeam Control A, Microbeam Control B, Microbeam sham 2 h A, Microbeam sham 2 h B, Microbeam sham 6 h A, Microbeam sham 6 h B, Microbeam 1c 2 h A, Microbeam 1c 2 h B, Microbeam 1c 6 h A, Microbeam 1c 6 h B, Microbeam 5c 2 h A, Microbeam 5c 2 h B, Microbeam 5c 6 h A, Microbeam 5c 6 h B, Microbeam 25c 2 h A, Microbeam 25c 2 h B, Microbeam 25c 6 h A, Microbeam 25c 6 h B.
Contributor(s) Iwakawa M, Hamada N, Imadome K
Citation(s) 18538798
Submission date Sep 09, 2007
Last update date Feb 22, 2018
Contact name Kaori Imadome
Organization name National institute of radiological sciences
Department Research Center of Charged Particle Therapy
Lab RadGenomics Project
Street address Anagawa 4-9-1
City Chiba
State/province Chiba
ZIP/Postal code 2658333
Country Japan
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (36)
GSM226663 Broadbeam control A
GSM226664 Broadbeam control B
GSM226665 Broadbeam sham 2 h A
BioProject PRJNA102465

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8993_RAW.tar 243.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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