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| Status |
Public on Sep 02, 2008 |
| Title |
Normal human fibroblast cell line bystander effects by Carbon ion irradiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We have recently investigated the response of irradiated and bystander fibroblasts to heavy ions in confluent cultures exposed randomly to broadbeam and selectively to precision microbeam that target a predefined fraction of cells with counted number of particle(s), respectively, and found the difference in the time course of apoptosis induction and p53 phosphorylation between irradiated and bystander cells [Hamada MR2008]. In the present investigation, to further scrutinize the underpinnings of their temporally distinct responses, we set out to examine the gene expression changes in irradiated and bystander fibroblasts at a genome-wide level with the microarray analysis under the same experimental condition. We demonstrate that the gene expression profiles of bystander cells are substantially different from those of heavy ion-irradiated cells.
Keywords: bystander effect, hour-time course cell number dependency, irradiation-dose response
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| Overall design |
36 samples ; Broadbeam control A, Broadbeam control B, Broadbeam sham 2 h A, Broadbeam sham 2 h B, Broadbeam sham 6 h A, Broadbeam sham 6 h B, Broadbeam D 2 h A, Broadbeam D 2 h B, Broadbeam D 6 h A, Broadbeam D 6 h B, Broadbeam 0.1D 2 h A, Broadbeam 0.1D 2 h B, Broadbeam 0.1D 6 h A, Broadbeam 0.1D 6 h B, Broadbeam 0.01D 2 h A, Broadbeam 0.01D 2 h B, Broadbeam 0.01D 6 h A, Broadbeam 0.01D 6 h B, Microbeam Control A, Microbeam Control B, Microbeam sham 2 h A, Microbeam sham 2 h B, Microbeam sham 6 h A, Microbeam sham 6 h B, Microbeam 1c 2 h A, Microbeam 1c 2 h B, Microbeam 1c 6 h A, Microbeam 1c 6 h B, Microbeam 5c 2 h A, Microbeam 5c 2 h B, Microbeam 5c 6 h A, Microbeam 5c 6 h B, Microbeam 25c 2 h A, Microbeam 25c 2 h B, Microbeam 25c 6 h A, Microbeam 25c 6 h B.
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| Contributor(s) |
Iwakawa M, Hamada N, Imadome K |
| Citation(s) |
18538798 |
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| Submission date |
Sep 09, 2007 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Kaori Imadome |
| Organization name |
National institute of radiological sciences
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| Department |
Research Center of Charged Particle Therapy
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| Lab |
RadGenomics Project
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| Street address |
Anagawa 4-9-1
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| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
2658333 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA102465 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8993_RAW.tar |
243.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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