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| Status |
Public on Nov 15, 2016 |
| Title |
BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis and male fertility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
BCAS2 (Breast cancer amplified sequence 2) is involved in multiple biological processes, including pre-mRNA splicing. However, the physiological roles of BCAS2 are still largely unclear. Here we report that BCAS2 is specifically enriched in spermatogonia of mouse testes. Conditional disruption of Bcas2 in male germ cells impairs spermatogenesis and leads to male mouse infertility. Although the spermatogonia appear grossly normal, spermatocytes in meiosis prophase I and meiosis events (recombination and synapsis) are rarely observed in the BCAS2-depleted testis. In BCAS2 null testis, 245 genes are altered in alternative splicing forms; at least three spermatogenesis-related genes (Dazl, Ehmt2 and Hmga1) can be verified. In addition, disruption of Bcas2 results in a significant decrease of the full-length form and an increase of the short form (lacking exon 8) of DAZL protein. Altogether, our results suggest that BCAS2 regulates alternative splicing in spermatogonia and the transition to meiosis initiation, and male fertility.
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| Overall design |
Transcriptional profiles of P9 testes from Control and Bcas2F/-;V-cre males
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| Contributor(s) |
Liu W, Li L |
| Citation(s) |
28128212 |
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| Submission date |
Nov 14, 2016 |
| Last update date |
Sep 11, 2019 |
| Contact name |
Wenbo Liu |
| E-mail(s) |
wenbo0324@163.com
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| Organization name |
Institute of zoology
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| Street address |
1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA353351 |
| SRA |
SRP093274 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89801_RAW.tar |
590.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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