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Series GSE89758 Query DataSets for GSE89758
Status Public on Feb 08, 2017
Title RNA-seq of Tumor-associated Endothelial Cells from Different Immunodeficient Backgrounds
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the impact of CD4+ T cells on tumor vasculature, we performed transcriptome profiling on tumor-associated endothelial cells in mice with or without functional CD4 T cells. In addition to examining four pathways that affect vessel maturation (VEGFA, ANGPT1/ANGPT2, TGFbR, and sphingolipid metabolism), we ran Gene Set Enrichment Analysis (GSEA) and found a down-regulation of cellular adhesion and extracellular matrix assembly-related pathways in the CD4 T cell deficient group. This suggests that CD4+ T cells play an important role in promoting tumor vessel integrity and normalization.
Overall design Transcriptome profiling of E0771 murine tumor-associated endothelial cells isolated from CD4+ T cell competent (CD8KO, Tie2Cre, WT) or deficient mouse strains (CD4KO, Tie2Cre;H2Ab flox and TCRKO) .
Contributor(s) Zhang X, Tian L, Wang H, Goldstein A
Citation(s) 28371798
Submission date Nov 10, 2016
Last update date May 15, 2019
Contact name Lin Tian
Phone 6468882063
Organization name Memorial Sloan Kettering Cancer Center
Department Cancer Biology & Genetics Program
Lab Joan Massagué
Street address Mortimer B. Zuckerman Research Center, 417 East 68th Street
City New York City
State/province New York
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM2388114 CD4KO_9768
GSM2388115 CD4KO_9769
GSM2388116 CD4KO_9770
BioProject PRJNA353109
SRA SRP093231

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89758_RAW.tar 38.1 Mb (http)(custom) TAR (of TXT)
GSE89758_VN.RData.gz 6.8 Mb (ftp)(http) RDATA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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