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Series GSE89532 Query DataSets for GSE89532
Status Public on Nov 30, 2016
Title RNAseq analysis of thymic CD4-CD8- double-negative (DN) invariant NKT cells from CD4+CD8+ double-positive (DP)-specific Rag2-conditional knock-out mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the identification of DN NKT cells developed from DN stage thymocytes. We analyzed the gene expression profiles of NKT cells that have developed from DN stage thymocytes isolated from DP-specific E8III-Cre transgenic Rag2-floxed mouse strain, and NKT cells developed from DP thymocytes sorted as YFP-reporter positive NKT cells that were isolated from E8III-Cre transgenic Rosa26-loxP-STOP-loxP mouse.
Overall design Next generation sequencing of total transcriptomes using total RNA isolated from FACS sorted ex vivo thymic and liver NKT cells. DN-origin NKT cells were obtained from DP-specific E8III-Cre transgenic Rag2-floxed mice, and DP-origin NKT cells were obtained from E8III-Cre transgenic Rosa26-loxP-STOP-loxP mouse. The sorting gate was determined as: 7AAD-CD45R-CD4-CD8-CD1d dimer+TCRb+ cells.
Contributor(s) Dashtsoodol N, Endo TA
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Submission date Nov 04, 2016
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (12)
GSM2375311 YFP+ NKT rep1
GSM2375312 YFP+ NKT rep2
GSM2375313 YFP+ NKT rep3
BioProject PRJNA352476
SRA SRP092590

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Supplementary file Size Download File type/resource
GSE89532_paired_rev2.txt.gz 354.7 Kb (ftp)(http) TXT
GSE89532_single_rev2.txt.gz 333.8 Kb (ftp)(http) TXT
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