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| Status |
Public on Jan 31, 2017 |
| Title |
PC1/3 deficiency impacts POMC processing in human embryonic stem cell-derived hypothalamic neurons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed a technique for generating hypothalamic neurons from human pluripotent stem cells. Here, as proof-of-principle, we examine the use of these cells in modeling of a monogenic form of severe obesity: PCSK1 deficiency. We generated PCSK1 (PC1/3)-deficient human embryonic stem cell (hESC) lines using both shRNA and CRISPR-Cas9, and investigated pro-opiomelanocortin (POMC) processing using hESC-differentiated hypothalamic neurons.
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| Overall design |
We tried to idenitify transcripitional profiles and specific transcription factors that involved in of different stages during hypothalamic neuron differentiation from single cell sequencing for hESC-derived Day27 hypothalamic neurons, Day 12 neuron progenitors and undifferentiated stem cells
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| |
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| Contributor(s) |
Wang L |
| Citation(s) |
28132887 |
| |
| Submission date |
Nov 03, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yurong Xin |
| E-mail(s) |
yurong.xin@regeneron.com
|
| Organization name |
Regeneron Pharmaceuticals, Inc.
|
| Street address |
777 Old Saw Mill River Road
|
| City |
Tarrytown |
| State/province |
NY |
| ZIP/Postal code |
10598 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (95)
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| Relations |
| BioProject |
PRJNA352463 |
| SRA |
SRP092584 |
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