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Series GSE89361 Query DataSets for GSE89361
Status Public on May 01, 2017
Title Genome-wide effect of STING-mediated activation in T cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using wild type mice and mice deficient for STING, we compared genome-wide expression of RNA in response to STING activation with DMXAA
Overall design To look for the role of STING-mediated pathway in activation of T cells, we activated naïve T cells with DMXAA, a synthetic agonist of STING, together with activation of TCR. To define a STING-specific effect, STING-deficient animals were used as a control. We isolated naïve T cells from peripheral lymph nodes, negatively selected them using magnetic beads coated with antibodies, and activated cells for 4 hours in specified conditions. Next, we isolated RNA and made library using TruSeq kit (Illumina) for the library construction.One replica of each sample is provided
Contributor(s) Poltorak A, Larkin B
Citation(s) 28615418
Submission date Oct 31, 2016
Last update date May 15, 2019
Contact name Alexander Poltorak
Phone 6176363596
Organization name Tufts University
Street address 150 Harrison avenue
City Boston
State/province Massachusetts
ZIP/Postal code 02111
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM2367173 C57BL6_Unstimulated
GSM2367174 STING-KO_Unstimulated
GSM2367175 C57BL6_TCR-activated
BioProject PRJNA351876
SRA SRP092352

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89361_genes.fpkm_tracking.gz 1.3 Mb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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