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| Status |
Public on May 01, 2017 |
| Title |
Genome-wide effect of STING-mediated activation in T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using wild type mice and mice deficient for STING, we compared genome-wide expression of RNA in response to STING activation with DMXAA
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| Overall design |
To look for the role of STING-mediated pathway in activation of T cells, we activated naïve T cells with DMXAA, a synthetic agonist of STING, together with activation of TCR. To define a STING-specific effect, STING-deficient animals were used as a control. We isolated naïve T cells from peripheral lymph nodes, negatively selected them using magnetic beads coated with antibodies, and activated cells for 4 hours in specified conditions. Next, we isolated RNA and made library using TruSeq kit (Illumina) for the library construction.One replica of each sample is provided
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| Contributor(s) |
Poltorak A, Larkin B |
| Citation(s) |
28615418 |
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| Submission date |
Oct 31, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Alexander Poltorak |
| Phone |
6176363596
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| Organization name |
Tufts University
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| Street address |
150 Harrison avenue
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02111 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA351876 |
| SRA |
SRP092352 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89361_genes.fpkm_tracking.gz |
1.3 Mb |
(ftp)(http) |
FPKM_TRACKING |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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