|
| Status |
Public on Mar 28, 2017 |
| Title |
Global analysis of gene expression reveals mRNA superinduction is required for the inducible immune response to a bacterial pathogen |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
The inducible innate immune response to infection requires a concerted process of gene expression that is regulated at multiple levels. Most global analyses of the innate immune response have focused on transcription induced by defined immunostimulatory ligands, such as lipopolysaccharide. However, the response to pathogens involves additional complexity, as pathogens interfere with virtually every step of gene expression. How cells respond to pathogen-mediated disruption of gene expression to nevertheless initiate protective responses remains unclear. We previously discovered that a pathogen-mediated blockade of host protein synthesis provokes the production of specific pro-inflammatory cytokines. It remains unclear how these cytokines are produced despite the global pathogen-induced block of translation. We addressed this question by using parallel RNAseq and ribosome profiling to characterize the response of macrophages to infection with the intracellular bacterial pathogen Legionella pneumophila. Our results reveal that mRNA superinduction is required for the inducible immune response to a bacterial pathogen.
|
| |
|
| Overall design |
Transcriptional and translational responses of bone marrow macrophages to infection with Legionella pneumophila were measured by RNAseq and ribosome profiling following standard protocols.
|
| |
|
| Contributor(s) |
Barry KC, Ingolia NT, Vance RE |
| Citation(s) |
28383283 |
| |
| Submission date |
Oct 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Barry |
| E-mail(s) |
Kevin.Barry@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Street address |
513 Parnassus Avenue Room HSW-518, Box 0511
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143-0511 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (41)
|
|
| Relations |
| BioProject |
PRJNA350544 |
| SRA |
SRP092069 |
Less...
More...