|
| Status |
Public on May 02, 2017 |
| Title |
Gene expression analysis of mouse embryonic fibroblasts reprogrammed with OSK, Esrrb and Zic3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We report that Zic family (Zic1/2/3) and orphan nuclear receptors family (Esrrb and Nr5a2) transcription factors (TFs) synergistically enhance the reprogramming efficiency when transduced with Oct4, Sox2 and Klf4 (OSK) into murine fibroblasts. To identify the molecular mechanisms underlying this synergy, we analyzed global gene expression at 6 days after introduction of reprogramming factors. As a result, we found that primary targets of these TFs are different when either of TFs was introduced with OSK, but a significant portion of genes including pluripotency makers such as Dppa2 was synergistically upregulated. Further analysis revealed that metabolic pathways are the important targets of these TFs for efficient reprogramming.
|
| |
|
| Overall design |
Mouse embryonic fibroblasts (MEFs) introduced with 6 different combinations of TFs with triplicates
|
| |
|
| Contributor(s) |
Sone M, Yamamoto T |
| Citation(s) |
28467928 |
| |
| Submission date |
Oct 24, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Takuya Yamamoto |
| Organization name |
Kyoto University
|
| Department |
CiRA
|
| Street address |
53 Kawahara-cho, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA350246 |
Less...
More...