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Status |
Public on Oct 22, 2016 |
Title |
Quant Seq analysis of primary stretched rat alveolar type I-like epithelial (AEC-I) cells |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Rat AEC-I cell monolayers were generated from primary isolated type-II pneumoncytes by plating them on fibronectin-coated silastic membranes. After 4 days of culturing, type-II pneumocytes trasform into AEC-I cells and form monolayers.
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Overall design |
Monolayers were randomized in the following four conditions: 1) unstretched monolayers (NS). 2) Monolayers exposed to 25% surface area change with custom built biaxial cyclic (15/min) stretcher for 6 hours (S). 3) Unstretched monolayers pretreated with 1microM GSK2606414 PERK kinase inhibitor for 18 hours (NS_PI). 4) Stretched monolayers pretreated with 1micro M GSK2606414 for 18 hours (S_PI).
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Contributor(s) |
Dolinay T, Shumyatcher M, Himes BE |
Citation(s) |
28363030 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
T32 HL007586 |
Training in Pulmonary Immunology |
UNIVERSITY OF PENNSYLVANIA |
Steven Mark Albelda |
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Submission date |
Oct 21, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Tamas Dolinay |
E-mail(s) |
tdolinay@hotmail.com
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Phone |
4125278764
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Organization name |
University of Pennsylvania
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Department |
Bioengineering
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Lab |
Susan Margulies
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Street address |
220 S. 33rd St., Towne building, Room 390
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City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
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Platforms (1) |
GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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Samples (20)
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Relations |
BioProject |
PRJNA349816 |
SRA |
SRP091911 |