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Series GSE88762 Query DataSets for GSE88762
Status Public on Oct 15, 2016
Title Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition [expression 2]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Epithelial to mesenchymal transition (EMT) in cancer cells has been associated with metastasis, stemness and resistance to therapy. The reason why some tumors undergo EMT and other not might reflect intrinsic properties of their cell of origin, although this possibility is largely unexplored. By targeting the same oncogenic mutations to discrete skin compartments, we show cell type-specific chromatin and transcriptional states differentially prime tumors to EMT. Squamous cell carcinomas (SCCs) derived from intrafollicular epidermis (IFE) are generally well-differentiated, while hair follicle (HF) stem cell-derived SCCs frequently exhibit EMT, efficiently form secondary tumors, and possess increased metastatic potential. Transcriptional and epigenomic profiling revealed IFE and HF tumor-initiating cells possess distinct chromatin landscapes and gene regulatory networks associated with tumorigenesis and EMT that correlate with accessibility of key epithelial and EMT transcription factor binding sites. These findings highlight the importance of chromatin states and transcriptional priming in dictating tumor phenotypes and EMT.


 
Overall design KrasG12D expression combined with deletion of p53 and overexpression of DeltaNp63 were induced in Hair Follicle Stem cells (SCs) and their progeny using Lgr5CREER/KRasG12D/p53fl/fl/DeltaNp53-IRES-GFP mice by intraperitoneal tamoxifen injection. Epcam+ tumors epithelial Cells and Epcam- mesenchymal tumor cells were FACS cells sorted and compared from fully grown tumours.
 
Contributor(s) Latil ML, Nassar D, Beck B, Boumhadi S, Brisebarre A, Blanpain C
Citation(s) 27889319
Submission date Oct 14, 2016
Last update date Aug 06, 2018
Contact name C├ędric Blanpain
E-mail(s) cedric.blanpain@ulb.ac.be
Organization name ULB
Street address Route de Lennik 808
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (10)
GSM2345153 EpnP63_1781
GSM2345154 EpnP63_1783
GSM2345155 EppP63_1780
This SubSeries is part of SuperSeries:
GSE71621 Cell type-specific chromatin states differentially prime squamous cell carcinoma tumor-initiating cells for epithelial to mesenchymal transition
Relations
BioProject PRJNA348520

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE88762_RAW.tar 21.8 Mb (http)(custom) TAR (of CEL)
GSE88762_log2_RMA_annotated.txt.gz 3.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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