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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 19, 2007 |
| Title |
Isolation and molecular characterization of cancer stem cells in MMTVWnt-1 murine breast tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In human breast cancers, a phenotypically distinct minority population of tumorigenic cancer (TG) cells (sometimes referred to as cancer stem cells) drives tumor growth when transplanted into immunodeficient mice. Our objective was to identify a mouse model of breast cancer stem cells that could have relevance to studying human breast cancer. To do so, we utilized breast tumors of the MMTVWnt-1 mice. MMTV-Wnt-1 breast tumors were harvested, dissociated into single cell suspensions, and FACS sorted on Thy1, CD24, and CD45. FACS sorted cells were then injected into recipient background FBV/NJ female mice. Thy1+CD24+ cancer cells, which constitute approximately 1-4% of tumor cells were highly enriched for cells capable of regenerating new tumors when compared to cells of the tumor that did not fit this profile (“Not Thy1+CD24+”). Resultant tumors were of the same phenotypic diversity as the original tumor and behaved in a similar manner when passaged. Microarray analysis comparing Thy1+CD24+ tumor cells to “Not Thy1+CD24+” cells identified a list of differentially expressed genes. Orthologs of these differentially expressed genes predicted survival of human breast cancer patients from two different study groups. These studies suggest that there is a cancer stem cell compartment in the MMTV-Wnt-1 murine breast tumor and that there is a clinical utility of this model for the study of cancer stem cells.
Keywords: cell type comparison
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| Overall design |
Expression profling were performed on 3 tumorigenic and 3 non tumorigenic samples of MMTV-Wnt-1 breast tumors. A gene signature was derived by comparing the gene expressions of 3 tumorigenic samples with 3 nontumorigenic samples
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| Contributor(s) |
Cho RW, Wang X, Diehn M, Shedden K, Chen GY, Sherlock G, Gurney A, Lewicki J, Clarke MF |
| Citation(s) |
17975224 |
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| Submission date |
Aug 20, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Xinhao Wang |
| E-mail(s) |
xinhao.wang@oncomed.com
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| Phone |
650-995-8211
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| Organization name |
OncoMed Pharmaceuticals Inc
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| Street address |
800 Chesapeake
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| City |
Redwood City |
| State/province |
CA |
| ZIP/Postal code |
94063 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM219325 |
tumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG1 |
| GSM219326 |
tumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG2 |
| GSM219327 |
tumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG3 |
| GSM219328 |
nontumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG1 |
| GSM219329 |
nontumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG2 |
| GSM219330 |
nontumorigenic population of MMTV-Wnt-1 breast tumor, Mouse TG3 |
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| Relations |
| BioProject |
PRJNA102157 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8828_RAW.tar |
20.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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