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| Status |
Public on Oct 12, 2016 |
| Title |
Expression data of siFAM210B and control A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Our previous study screened a novel cancer progression suppressor gene, FAM210B, which encodes an outer mitochondrial membrane protein, by the suppression of mortality by antisense rescue technique (SMART). We demonstrated that FAM210B loss was significantly associated with cancer metastasis and decreased survival in a clinical setting.
We used microarrays to find diffrenet gene expression between siFAM210B and control A549 cells
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| Overall design |
A549 cells were transfected with siRNA for 72h for RNA extraction and hybridization on Affymetrix microarrays, and Realtime PCR were used to verified the effect of siRNA knockdown. We used mixed siRNA, tirplicated per sample and designed the control to increase the resolution of expression profiles.
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| Contributor(s) |
Sun S, Liu J |
| Citation(s) |
30674441 |
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| Submission date |
Oct 11, 2016 |
| Last update date |
Mar 28, 2019 |
| Contact name |
Shujuan Sun |
| E-mail(s) |
ssj200975831@163.com
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| Organization name |
Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
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| Street address |
Cancer Biology Research Center, Tongji Hospital,Tongji Medical College, Huazhong University of Science and Technology, 1095 JiefangAnv, Wuhan,China
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| City |
Wuhan |
| ZIP/Postal code |
430030 |
| Country |
China |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA347859 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87850_RAW.tar |
215.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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