|
| Status |
Public on Feb 28, 2017 |
| Title |
Genomic integration of Wnt/b-catenin and BMP/Smad1 coordinates the transcriptional program of foregut and hindgut progenitors [RNA-seq] |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Digestive system development is orchestrated by combinatorial signaling interactions between endoderm and mesoderm, but how they are integrated in the genome is poorly understood. Here we identified the Xenopus foregut and hindgut progenitor transcriptomes, which are largely conserved with mammals. Using RNA-seq and ChIP-seq we show that BMP/Smad1 regulates dorsal-ventral gene expression in both the endoderm and mesoderm, whereas Wnt/b-catenin acts as a genome-wide toggle between foregut and hindgut programs. In addition to b-catenin-Tcf promoting hindgut gene transcription, we unexpectedly observed Wnt-repressed foregut genes associated with b-catenin-binding to DNA lacking Tcf motifs, suggesting a novel direct repression. We define how BMP and Wnt signaling are integrated in the genome with Smad1 and β-catenin co-occupying DNA elements associated with hundreds of key regulatory genes. These results extend our understanding of GI organogenesis and how Wnt and BMP may coordinate genomic responses in other contexts.
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| |
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| Overall design |
mRNA profile of foregut and hindgut explants from BMP and Wnt manipulated embryos.
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| Contributor(s) |
Stevens ML, Chaturvedi P, Rankin SA, Macdonald M, Jagannathan S, Yukawa M, Barski A, Zorn AM |
| Citation(s) |
28219948 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK070858 |
Molecular Basis of Liver Development: Molecular Basis of Liver Development.: Molecular Basis of Liver Development.: Molecular basis of digestive system development in Xenopus: Molecular basis of digestive system development in Xenopus: Molecular basis of digestive system development in Xenopus |
CHILDREN'S HOSPITAL MEDICAL CENTER |
Aaron M Zorn |
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| |
| Submission date |
Oct 05, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Aaron M Zorn |
| E-mail(s) |
aaron.zorn@cchmc.org
|
| Phone |
636 3770
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Develpomental Biology
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45236 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18936 |
Illumina HiSeq 2500 (Xenopus laevis) |
|
| Samples (37)
|
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| This SubSeries is part of SuperSeries: |
| GSE87654 |
Genomic integration of Wnt/b-catenin and BMP/Smad1 coordinates the transcriptional program of foregut and hindgut progenitors |
|
| Relations |
| BioProject |
PRJNA345496 |
| SRA |
SRP090889 |
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