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Series GSE87476 Query DataSets for GSE87476
Status Public on Sep 20, 2017
Title Mitotic Transcription and Waves of Gene Reactivation During Mitotic Exit
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The genome is thought to be transcriptionally silent during mitosis. Technical limitations have prevented the sensitive mapping of mitotic transcription and transcription reactivation during mitotic exit, and thus the networks by which transcriptome dynamics govern the generation of daughter cells have been unclear. We used 5-ethynyluridine to pulse-label intact transcripts during mitosis and mitotic exit and find that the first round of transcription activates genes that are involved in macromolecular synthesis, rather than cell identity. Many interphase genes exhibit residual transcription in mitosis, as confirmed by different methods of assessment, and a subset of genes are more highly transcribed in mitosis than in interphase. We suggest that mitotic transcription may serve an epigenetic function in restoring proper transcription patterns during mitotic exit.
Overall design All samples in triplicates A, B, and C; experimental time points = 0, 40, 80, 105, 165, 300, EU; No label control = NoEU. Samples in triplicates D, E, F: mitotic, async; samples in duplicate: NoEU D, E.
Contributor(s) Palozola KC, Donahue G, Grant GR, Becker JS, Cote A, Liu H, Yu H, Raj A, Zaret KS
Citation(s) 28912132
Submission date Sep 29, 2016
Last update date Jul 25, 2021
Contact name Katherine Claire Palozola
Organization name University of Pennsylvania
Department Cell and Developmental Biology
Lab Kenneth S. Zaret
Street address 3400 Civic Center Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (32)
GSM2332392 0A
GSM2332393 0B
GSM2332394 0C
BioProject PRJNA344882
SRA SRP090620

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Supplementary file Size Download File type/resource
GSE87476_RAW.tar 22.5 Gb (http)(custom) TAR (of BW)
GSE87476_palozola_FPKMs.txt.gz 1.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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