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| Status |
Public on Jan 21, 2019 |
| Title |
Myc activation in breast cancer is due to p53-loss and sustains self-renewal of cancer stem cells (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: discover the downstream pathways and the mechanism of target activation by the p53:Myc axis in normal mammary and cancer stem cells
Methods: we established mammosphere culture from normal and tumoral murine mammary glands and extracted RNA for expression analysis at passage 3 of the serial replating assay
Results: These data demonstrate the existence of a set of 189 p53 and Myc common targets, which are implicated in the regulation of mitosis and are up-regulated in the ErbB2-tumor mammospheres, and suggest that these genes are crucial for the regulation of CSC numbers.
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| Overall design |
Examination of expression profile in mammospheres derived from WT, L-MyCER, ErbB2-tumor and p53-/- murine mammary cells
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| Contributor(s) |
Santoro A, Vlachou T, Luzi L, Pasi C, Reavie L, Melloni G, Bonetti P, Pelicci PG |
| Citation(s) |
30650356 |
| |
| Submission date |
Sep 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Pier Giuseppe Pelicci |
| Organization name |
European Institute of Oncology
|
| Department |
Department of Experimental Oncology
|
| Street address |
Via Adamello, 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE87004 |
Myc activation in breast cancer is due to p53-loss and sustains self-renewal of cancer stem cells |
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| Relations |
| BioProject |
PRJNA343202 |
| SRA |
SRP089998 |
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