|
| Status |
Public on Dec 31, 2018 |
| Title |
Infiltrating monocyte-derived macrophages polarizes in to anti-inflammatory and neuro-protective phenotype in a rat model of cerebral ischemia |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To summarize our results, we found microglia and MDMs are functionally heterogeneous population contributing differently to the disease phenotype. Our data, suggest that during post-ischemic inflammation microglia exhibit pro-inflammatory phenotype, while infiltrating MDMs display anti-inflammatory and neuro-protective phenotype. Apart from up-regulation of some of the classical M2 marker in MDMs, our gene expression profiling using RNA-sequencing revealed a unique signature of MDMs with the up-regulation of genes associated with wound healing, MHCII antigen-presentation and tissue repair. Our results strongly suggest that MDMs infiltrating the CNS parenchyma during the sub-acute of post-ischemic inflammation might contribute to the endogenous mechanism of neuroprotection.
|
| |
|
| Overall design |
RNAseq profilling of sorted microglia and macrophages after MCAO compared to sham
|
| |
|
| Contributor(s) |
Wojtas B, Karunakaran W |
| Citation(s) |
30485549 |
| |
| Submission date |
Sep 14, 2016 |
| Last update date |
Jun 24, 2019 |
| Contact name |
Bartosz Wojtas |
| E-mail(s) |
bartoszwojtas83@gmail.com
|
| Phone |
692156006
|
| Organization name |
Nencki Institute of Experimental Biology
|
| Street address |
Pasteura 3
|
| City |
Warsaw |
| ZIP/Postal code |
02-793 |
| Country |
Poland |
| |
|
| Platforms (1) |
| GPL18404 |
Illumina HiSeq 1500 (Rattus norvegicus) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA343038 |
| SRA |
SRP089894 |
Less...
More...