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| Status |
Public on Jul 01, 2017 |
| Title |
Dermal fibroblasts play a central role in skin model protection against C. albicans invasion |
| Organisms |
Homo sapiens; Candida albicans SC5314 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study shows that, in the presence of CD4+ T cells, dermal fibroblasts shift towards an antimicrobial phenotype leading to reduced dermal invasion by C. albicans. This is dependent on TLR2 activation and pro-IL-1β cleavage and results in expression of genes involved in defense against various classes of pathogens.
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| Overall design |
Dual RNA-Seq of keratinocytes and fibroblasts with or without Candida albicans from infected humen reconstituted skin models with or without CD4+ T cells. Sequenced were fibroblasts, Keratinocytes and C. albicans isolated from these models. For each sample biological triplicates were analyzed.
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| |
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| Contributor(s) |
Kühbacher A, Stevens P, Grumaz C, Henkel H, Finkelmeier D, Burger-Kentischer A, Sohn K, Rupp S |
| Citation(s) |
28368492 |
| |
| Submission date |
Sep 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian Grumaz |
| Organization name |
Fraunhofer IGB
|
| Department |
MBT
|
| Street address |
Nobelstr. 12
|
| City |
Stuttgart |
| ZIP/Postal code |
70569 |
| Country |
Germany |
| |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL22432 |
Illumina HiSeq 2500 (Candida albicans SC5314; Homo sapiens) |
|
| Samples (18)
|
| GSM2310377 |
Fibroblasts infected Human and Candida BR1 |
| GSM2310378 |
Fibroblasts infected Human and Candida BR2 |
| GSM2310379 |
Fibroblasts infected Human and Candida BR3 |
| GSM2310380 |
Keratinocytes uninfected Human BR1 |
| GSM2310381 |
Keratinocytes uninfected Human BR2 |
| GSM2310382 |
Keratinocytes uninfected Human BR3 |
| GSM2310383 |
Keratinocytes infected Human and Candida BR1 |
| GSM2310384 |
Keratinocytes infected Human and Candida BR2 |
| GSM2310385 |
Keratinocytes infected Human and Candida BR3 |
| GSM2310386 |
Fibroblasts infected Human and Candida no T cells BR1 |
| GSM2310387 |
Fibroblasts infected Human and Candida no T cells BR2 |
| GSM2310388 |
Fibroblasts infected Human and Candida no T cells BR3 |
| GSM2310389 |
Fibroblasts infected Human and Candida with T cells BR1 |
| GSM2310390 |
Fibroblasts infected Human and Candida with T cells BR2 |
| GSM2310391 |
Fibroblasts infected Human and Candida with T cells BR3 |
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| Relations |
| BioProject |
PRJNA343010 |
| SRA |
SRP089880 |
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