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Series GSE86865 Query DataSets for GSE86865
Status Public on May 15, 2017
Title RNA-seq transcriptonal profiling in E13.5 fetal liver erythroid cells from Tfam WT and KO mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The developing erythroid cells require highly coordinated gene expression and metabolism. By comparing the proteomic and transcriptomic changes in human hematopoietic stem/progenitor cells (HSPCs) and lineage-committed erythroid progenitors (ProEs), and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. Two principal mitochondrial factors TFAM and PHB2 are tightly regulated at the protein level and indispensable for mitochondria and erythropoiesis. To determine the role of TFAM in mitochondrial function during erythroid development, we generated Tfam conditional knockout (KO) mice by an erythroid-specific EpoR-Cre allele. We isolated the CD71+Ter119+ embryonic day (E)13.5 fetal liver erythroid cells by FACS sorting, and performed RNA-seq transcriptional profiling analysis.
Overall design CD71+Ter119+ embryonic day (E)13.5 fetal liver erythroid cells were isolated by FACS sorting. Total RNA were extracted and processed for RNA-seq transcriptional profiling analysis.
Contributor(s) Liu X, Zhang Y, Shao Z, Xu J
Citation(s) 28504707
Submission date Sep 13, 2016
Last update date May 15, 2019
Contact name Jian Xu
Phone 214-648-6125
Organization name UT Southwestern Medical Center
Department Children's Research Institute
Street address 6000 Harry Hines Blvd. NL12.138B
City Dallas
State/province TX
ZIP/Postal code 75235
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2309714 RNAseq_Tfam-WT1
GSM2309715 RNAseq_Tfam-WT2
GSM2309716 RNAseq_Tfam-WT3
This SubSeries is part of SuperSeries:
GSE86912 Quantitative Proteomic and Transcriptomic Analysis Reveals Post-Transcriptional Regulation of Mitochondrial Biogenesis during Erythropoiesis
BioProject PRJNA342800
SRA SRP089800

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86865_RNA-seq_Tfam_WTvsKO_RPKM.txt.gz 413.3 Kb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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