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Status |
Public on Sep 06, 2020 |
Title |
Global gene expression pattern in human first trimester primary villous cytotrophoblast cells (vCTBs) in comparison with human first trimester placental villous mesenchymal cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
vCTBs and mesenchymal cells were isolated from palcental villi of medically terminated and deidentified first trimester placenta (pulled samples from 4-6 placentas of 8-10 week gestation).
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Overall design |
Proliferating vCTBs and mesenchymal cells were isolated through enzymatic digestion followed by a percoll gradient separation and hence cultured in a specilized medium (TM, #7121, ScienCell Research Laboratories). RNA-seq analysis were performed using a illumina HiSeq2500 platform.
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Contributor(s) |
Paul S, Saha B, Gunewardena S |
Citation missing |
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Submission date |
Sep 12, 2016 |
Last update date |
Sep 06, 2020 |
Contact name |
Sumedha S Gunewardena |
Phone |
9139456878
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Organization name |
University of kansas medical center
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Department |
Dept. of Molecular and Integrative Physiology
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Street address |
2146 West 39th Avenue
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City |
Kansas City |
State/province |
Kansas |
ZIP/Postal code |
66160 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (2) |
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Relations |
BioProject |
PRJNA342592 |
SRA |
SRP089674 |